Immune Response of Ross 308 Broiler Chicken (Gallus gallus domesticus) Fed with Brown Seaweed (Laminaria spp.)
DOI:
https://doi.org/10.5281/zenodo.23383078Keywords:
Broiler chicken, brown seaweed, immune response, antibiotic growth promotersAbstract
The study documented the immune response of Ross 308 broiler chickens fed 2% brown seaweed (Laminaria spp.) as an alternative to antibiotic growth promoters during early post-hatch and grower-finisher stages. A completely randomized design with a 3 (post-hatch) x 2 (grower-finisher) diet in factorial arrangement was employed. A total of 792 viable embryos were utilized for the post-hatch trial, which lasted for 24 hours in the hatcher; the diet included PH1 (control/ no feed and water), PH2 (standard starter and water), and PH3 (standard starter with 2%BS and water), each with three replicates. Grower-finisher feeding used 576 chicks from hatcher and randomized in 2 dietary treatments mainly GF1 (standard diet) and GF2 (standard diet with 2%BS) that spanned for 35 days under controlled room temperature. Post-hatch and grower-finisher feeding of 2%BS did not significantly (p>0.05) affect the leukocyte cell count and H/L ratio of birds. The post-hatch H/L ratio of birds fed with PH3 (0.4330) was the lowest. However, birds fed with PH3 were observed to have the highest basophil cell count (1.44%). The result showed that grower-finisher feeding of 2%BS indicated a lower H/L ratio (0.4184) in contrast with the control. GF2 feeding elevated the basophil count (1.50%) and significantly (p=0.0286) increased the population of monocytes. The findings suggest that 2% brown seaweed improved immune response of birds.
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Abou-Jaoudeh, C., Andary, J., & Abou-Khalil, R. (2024). Antibiotic residues in poultry products and bacterial resistance: A review in developing countries. Journal of Infection and Public Health, 17(12), Article 102592. https://doi.org/10.1016/j.jiph.2024.102592
Abreu, R., Semedo-Lemsaddek, T., Cunha, E., Tavares, L., & Oliveira, M. (2023). Antimicrobial drug resistance in poultry production: Current status and innovative strategies for bacterial control. Microorganisms, 11(4), Article 953. https://doi.org/10.3390/microorganisms11040953
Al-Khalaifah, H. S., Al-Nasser, A., & Surrayai, T. (2022). Effects from dietary addition of Sargassum sp., Spirulina sp., or Gracilaria sp. powder on immune status in broiler chickens. Frontiers in Veterinary Science, 9, Article 928235. https://doi.org/10.3389/fvets.2022.928235
Dal Pont, G. C., Belote, B. L., Lee, A., Bortoluzzi, C., Eyng, C., Sevastiyanova, M., & Kogut, M. H. (2021). Novel models for chronic intestinal inflammation in chickens: Intestinal inflammation pattern and biomarkers. Frontiers in Immunology, 12, Article 676628. https://doi.org/10.3389/fimmu.2021.676628
El-Deek, A. A., Al-Harthi, M. A., Abdalla, A. A., & Elbanoby, M. M. (2011). The use of brown algae meal in finisher broiler diets. Egyptian Poultry Science, 31(3), 767–781.
El-Fateh, M., Bilal, M., & Zhao, X. (2024). Effect of antibiotic growth promoters (AGPs) on feed conversion ratio (FCR) of broiler chickens: A meta-analysis. Poultry Science, 103(12), Article 104472. https://doi.org/10.1016/j.psj.2024.104472
El-Hack, M. E., El-Saadony, M. T., Elbestawy, A. R., El-Shall, N. A., Saad, A. M., Salem, H. M., & El-Tarabily, K. A. (2022). Necrotic enteritis in broiler chickens: Disease characteristics and prevention using organic antibiotic alternatives—A comprehensive review. Poultry Science, 101(2), Article 101590. https://doi.org/10.1016/j.psj.2021.101590
Gadde, U., Kim, W. H., Oh, S. T., & Lillehoj, H. S. (2017). Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: A review. Animal Health Research Reviews, 18(1), 26–45. https://doi.org/10.1017/S146625231600020X
Gu, H. Y., Liu, Y. G., & Shu, Z. Z. (1988). Nutrient composition of marine algae and their feeding effects on broilers. Chinese Journal of Animal Science, 24(3), 12–15.
Hernández-Granados, M. J., Ortiz-Basurto, R. I., Jiménez-Fernández, M., García-Munguía, C. A., & Franco-Robles, E. (2021). Dietary encapsulated Bifidobacterium animalis and Agave fructans improve growth performance, health parameters, and immune response in broiler chickens. Animal Bioscience, 35(4), 587–598. https://doi.org/10.5713/ab.21.0115
Kim, G. B., Seo, Y. M., Kim, C. H., & Paik, I. K. (2011). Effect of dietary prebiotic supplementation on the performance, intestinal microflora, and immune response of broilers. Poultry Science, 90(1), 75–82. https://doi.org/10.3382/ps.2010-00944
Li, Y., Zheng, Y., Zhang, Y., Yang, Y., Wang, P., Imre, B., & Wang, D. (2021). Brown algae carbohydrates: Structures, pharmaceutical properties, and research challenges. Marine Drugs, 19(11), Article 620. https://doi.org/10.3390/md19110620
Maxwell, M. H., Robertson, G. W., Spence, S., & McCorquodale, C. C. (1990). Comparison of haematological values in restricted- and ad libitum-fed domestic fowls: White blood cells and thrombocytes. British Poultry Science, 31(2), 399–405. https://doi.org/10.1080/00071669008417272
Naeem, M. (2026). Combating necrotic enteritis in poultry through nutritional interventions: A review of alternatives to antibiotics. Animal Diseases, 6(1), Article 16. https://doi.org/10.1186/s44149-026-00184-y
Niewold, T. A. (2007). The nonantibiotic anti-inflammatory effect of antimicrobial growth promoters, the real mode of action? A hypothesis. Poultry Science, 86(4), 605–609. https://doi.org/10.3382/ps.2006-00232
Philippine Statistics Authority. (2026). Livestock and poultry quarterly bulletin: Volume 3, no. 4 (October–December 2025). Philippine Statistics Authority.
Rivera-Pérez, W., Barquero-Calvo, E., & Chaves, A. J. (2021). Effect of the use of probiotic Bacillus subtilis (QST 713) as a growth promoter in broilers: An alternative to bacitracin methylene disalicylate. Poultry Science, 100(9), Article 101372. https://doi.org/10.1016/j.psj.2021.101372
Sabah Abdulameer, Y. (2018). The study of different growth promoters on growth performance, intestinal bacteriology and haematological characteristics. Journal of Pure and Applied Microbiology, 12(4), 2111–2117. https://doi.org/10.22207/JPAM.12.4.45
Sayed, Y., Hassan, M., Salem, H. M., Al-Amry, K., & Eid, G. E. (2023). Prophylactic influences of prebiotics on gut microbiome and immune response of heat-stressed broiler chickens. Scientific Reports, 13(1), Article 13991. https://doi.org/10.1038/s41598-023-41221-5
Sweeney, T., Meredith, H., Ryan, M. T., Gath, V., Thornton, K., & O'Doherty, J. V. (2016). Effects of Ascophyllum nodosum supplementation on Campylobacter jejuni colonisation, performance and gut health following an experimental challenge in 10-day old chicks. Innovative Food Science & Emerging Technologies, 37, 247–252. https://doi.org/10.1016/j.ifset.2016.03.011
Tang, S. G. H., Sieo, C. C., Ramasamy, K., Saad, W. Z., Wong, H. K., & Ho, Y. W. (2017). Performance, biochemical and haematological responses, and relative organ weights of laying hens fed diets supplemented with prebiotic, probiotic and synbiotic. BMC Veterinary Research, 13(1), Article 248. https://doi.org/10.1186/s12917-017-1174-8
Tayeri, V., Seidavi, A., Asadpour, L., & Phillips, C. J. (2018). A comparison of the effects of antibiotics, probiotics, synbiotics and prebiotics on the performance and carcass characteristics of broilers. Veterinary Research Communications, 42(3), 195–207. https://doi.org/10.1007/s11259-018-9721-5
Wamboi, P., Waruiru, R. M., Mbuthia, P. G., Nguhiu, J. M., & Bebora, L. C. (2020). Haemato-biochemical changes and prevalence of parasitic infections of indigenous chicken sold in markets of Kiambu County, Kenya. International Journal of Veterinary Science and Medicine, 8(1), 18–25. https://doi.org/10.1080/23144599.2020.1774357
Wassie, T., Lu, Z., Duan, X., Xie, C., Gebeyew, K., Zhang, Y., & Wu, X. (2021). Dietary Enteromorpha polysaccharide enhances intestinal immune response and microbial activity in broilers. Frontiers in Nutrition, 8, Article 783819. https://doi.org/10.3389/fnut.2021.783819
Wati, T., Ghosh, T. K., Syed, B., & Haldar, S. (2015). Comparative efficacy of a phytogenic feed additive and an antibiotic growth promoter on production performance, caecal microbial population and humoral immune response of broiler chickens inoculated with enteric pathogens. Animal Nutrition, 1(3), 213–219. https://doi.org/10.1016/j.aninu.2015.08.008
Zanu, H. K., Kheravii, S. K., Morgan, N. K., Bedford, M. R., & Swick, R. A. (2020). Over-processed meat and bone meal and phytase effects on broilers challenged with subclinical necrotic enteritis: Part 2. Inositol phosphate esters hydrolysis, intestinal permeability, hematology, jejunal gene expression and intestinal morphology. Animal Nutrition, 6(4), 488–498. https://doi.org/10.1016/j.aninu.2020.08.006
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