Light Microscopy Analysis of Algal Diversity and Abundance in the Freshwater Ecosystem of Lake Sebu, South Cotabato
DOI:
https://doi.org/10.5281/zenodo.19463400Keywords:
Algae, Freshwater, Lake Sebu, Biodiversity, Abundance, Diversity IndicesAbstract
This study aimed to identify and classify algae, determine their abundance and distribution, and provide baseline data for ecological monitoring. Using a descriptive research design and light microscopy, samples were collected from four stations Falls 1, Falls 2, Lake Sebu (East), and Lake Sebu (West). Results revealed that green algae were the most dominant group, followed by filamentous green algae and diatoms. The computed indices, the Shannon (H= 0.89), Evenness (E = 0.66), and Dominance (D = 0.50) indicate moderate diversity and balanced distribution. These findings suggest that Lake Sebu supports a mesotrophic freshwater ecosystem, characterized by moderate nutrient levels and ecological stability. Overall, the study provides essential baseline data for understanding algal composition, assessing water quality and supporting the sustainable management and conservation of Lake Sebu's freshwater resources. It is recommended that future studies include seasonal sampling, consult a statistician and secure an accessible laboratory in the area to ensure timely observation, improved accuracy, and long-term monitoring of Lake Sebu’s algal diversity and ecological health.
Downloads
References
Admin. (2025, July 12). Bioindicators Nature’s Early Warning System – Green Metric. https://greenmetric.uma.ac.id/2025/07/12/bioindicators-natures-early-warning-system/
Ahmed, S. (2025). Algae as bioindicators of aquatic pollution. Randolph. https://www.academia.edu/142975950/Algae_as_Bioindicators_of_Aquatic_Pollution
Arora, M., & Sahoo, D. (2015). Concepts and techniques for the study of algae. In Cellular origin and life in extreme habitats (pp. 519–553). https://doi.org/10.1007/978-94-017-7321-8_21
Astronot. (2025, March 22). Photosynthesis in Algae: Fueling Aquatic life. Scienceinsider.blog. https://scienceinsider.blog/photosynthesis-algae-aquatic-life/
Biobide. (2024, June 11). Algae: Understanding its role in the ecosystem. Biobide. https://biobide.com/what-is-algae-and-uses
Biology Online. (2024b, January 25). Microalgae Definition and Examples - Biology Online Dictionary. Biology Articles, Tutorials & Dictionary Online. https://www.biologyonline.com/dictionary/microalgae
Briceño, G., V. (2021, December 2). Fresh water | What is it, characteristics, types, how it is produced, uses, sources. Euston96. https://www.euston96.com/en/fresh-water/
Freshwater-Aquaculture. (2019, August 26). If algae produce oxygen in a pond, how can having too many algae cause oxygen depletion? – Freshwater Aquaculture. https://freshwater-aquaculture.extension.org/if-algae-produce-oxygen-in-a-pond-how-can-having-too-much-algae-cause-an-oxygen-depletion/
Bobbitt, Z. (2021, March 29). Simpson’s Diversity Index: Definition & Examples. Statology. https://www.statology.org/simpsons-diversity-index/
Bobbitt, Z. (2022, April 20). Shannon Diversity Index: Definition & example. Statology. https://www.statology.org/shannon-diversity-index/
Helmenstine, A. (2025, August 13). Algae - Definition, Examples, Characteristics. Science Notes and Projects. https://sciencenotes.org/algae-definition-examples-characteristics/
Howard, D. (2023, October 20). How does algae contribute to the ocean food webs? – TravelAsker. TravelAsker. https://travelasker.com/how-does-algae-contribute-to-the-ocean-food-webs/
Johnson, A. (2022, September 8). What are biological indicators? Temporary. https://scienceoxygen.com/what-are-biological-indicators/
Kiprop, J. (2018, September 28). What is the ecological importance of algae? WorldAtlas. https://www.worldatlas.com/articles/what-is-the-ecological-importance-of-algae.html
Pomelo. (2025, July 30). Do algae produce oxygen? | Atlas Scientific. Atlas Scientific. https://atlas-scientific.com/blog/does-algae-produce-oxygen/
Team, M. (2021, February 26). What are macroalgae and how are they useful? - My reef. My Reef. https://myreef.aquariumdepot.com/what-are-macroalgae-and-how-are-they-useful/
Technology, L. (2025, July 7). Importance of lakes in ecosystems: vital freshwater source.Life TechnologyTM. https://www.lifetechnology.com/blogs/life-technology-science-news/importance-of-lakes-in-ecosystems-vital-freshwater-source
T, N. (2016, August 24). Algae: Definition, Characteristics and Structure (With Diagram). Biology Discussion. https://www.biologydiscussion.com/algae/algae-definition-characteristics-and-structure-with-diagram/46727
Young, J. N., Anderson, R., Martiny, A. C., & Morel, F. M. (2020). Physiological adaptations of high‐ latitude marine microalgae to environmental change. New Phytologist, 226(2), 366–372. https://doi.org/10.1111/nph.16648
Adhiambo, R., Mensah, P. K., & Acheampong, E. (2023). Widespread geographical disparities in phytoplankton ecology research in the face of climate change: A review. Water, 15(24), 4288. https://doi.org/10.3390/w15244288
Anderson, C. R., Berdalet, E., Kudela, R. M., Cusack, C. K., Silke, J., O’Rourke, E., Dugan, D., McCammon, M., Newton, J. A., Moore, S. K., Paige, K., Ruberg, S., Morrison, J. R., Kirkpatrick, B., Hubbard, K., Morell, J. (2019). Scaling up from regional case studies to a global harmful algal bloom observing system. Frontiers in Marine Science, 6, 250. https://doi.org/10.3389/fmars.2019.00250
Brenckman, C., Jayalakshmamma, A. P., Pennock, C., Ashraf, M., & Borgaonkar, H. (2025). A review of harmful algal blooms: Causes, effects, monitoring, and prevention methods. Water, 17(13), 1980. https://doi.org/10.3390/w17131980
Carey, R. O., Hochmuth, G. J., Martinez, C. J., Boyer, T. H., Dukes, M. D., & Toor, G. S. (2021). A review of water quality responses to air temperature and precipitation changes 2: Nutrients, algal blooms, sediment, pathogens. Environmental Science & Technology, 55(1), 12–30. https://doi.org/10.1021/acs.est.0c02470
Chan, Y., Chiang, K., Ku, Y., & Gong, G. (2018). Abiotic and biotic factors affecting the ingestion rates of mixotrophic nanoflagellates (Haptophyta). Microbial Ecology, 77(3), 607–615. https://doi.org/10.1007/s00248-018-1249-2
Chekanov, K. A., & Solovchenko, A. E. (2015). Possibilities and limitations of non-destructive monitoring of the unicellular green microalgae (Chlorophyta) in the course of balanced growth. Russian Journal of Plant Physiology, 62(2), 270–278. https://doi.org/10.1134/s1021443715010033
Chandel, P., Mahajan, D., Thakur, K., Kumar, R., Kumar, S., Brar, B., Sharma, D., & Sharma, A. K. (2023). A review on plankton as a bioindicator: A promising tool for monitoring water quality. World Water Policy, 10(1), 213–232. https://doi.org/10.1002/wwp2.12137
Galloway, A. W. E., Britton‐Simmons, K. H., Duggins, D. O., Gabrielson, P. W., & Brett, M. T. (2012). Fatty acid signatures differentiate marine macrophytes at ordinal and family ranks. Journal of Phycology, 48(4), 956–965. https://doi.org/10.1111/j.1529-8817.2012.01173.x
Gu, X., Cao, Z., Zhao, L., Seswita-Zilda, D., Zhang, Q., Fu, L., & Li, J. (2024). Application of environmental DNA metabarcoding to differentiate algal communities by littoral zonation and detect unreported algal species. Phycology, 4(4), 605–620. https://doi.org/10.3390/phycology4040033
Harun, R., Singh, M., Forde, G. M., & Danquah, M. K. (2013). Biochemical composition of algae influenced by environmental conditions: A review. Energies, 6(9), 4607–4638. https://doi.org/10.3390/en6094607
Islam, S. T., Dar, S. A., Sofi, M. S., Bhat, S. U, Sabha, I., Hamid, A., Jehangir, A., Bhat, A. A. (2021). Limnochemistry and plankton diversity in some high-altitude lakes of Kashmir Himalaya. Frontiers in Environmental Science, 9, 681965. https://doi.org/10.3389/fenvs.2021.681965
Jacobs, P., Serre‐Fredj, L., Koeman, R. P. T., Van Den Oever, A., Peck, M. A., & Philippart, C. J. M. (2024). Impacts of counting protocols for light microscopy on estimates of biodiversity and algal density of phytoplankton. Limnology and Oceanography Methods. https://doi.org/10.1002/lom3.10651
Jansen, H. M., Bernard, M. S., Marit, Ingrid, & van. (2022). Seasonal variation in productivity, chemical composition and nutrient uptake of Ulva spp. (Chlorophyta) strains. Journal of Applied Phycology, 34(3), 1649–1660. https://doi.org/10.1007/s10811-022-02708-z
Jia, J., Chen, Q., Ren, H., Lu, R., He, H., & Gu, P. (2022). Phytoplankton Composition and Their Related Factors in Five Different Lakes in China: Implications for Lake Management. 19(5), 3135–3135. https://doi.org/10.3390/ijerph19053135
Kawai, H., & Motomura, T. (2024). Structural colour in the brown algal genus Sporochnus (Sporochnales, Phaeophyceae). European Journal of Phycology, 59(3), 271–278. https://doi.org/10.1080/09670262.2024.2340020
Khalil, S., Mahnashi, M. H., Hussain, M., Zafar, N., Waqar-Un-Nisa, N., Khan, F. S., Afzal, U., Shah, G. M., Niazi, U. M., Awais, M., & Irfan, M. (2021). Exploration and determination of algal role as bioindicator to evaluate water quality – Probing freshwater algae. Saudi Journal of Biological Sciences, 28(10), 5728–5737. https://doi.org/10.1016/j.sjbs.2021.06.004
Kumar, M., Singh, D. P., Prabha, R., & Sharma, A. K. (2014). Role of cyanobacteria in nutrient cycle and use efficiency in the soil. In Springer eBooks (pp. 163–171). https://doi.org/10.1007/978-81-322-2169-2_10
Lestari, N., Kusuma, L., Yuliana, N., & Noerdjito, D. R. (2025). Biodiversity and distribution of freshwater microalgae from the black-water ecosystem of Rungan River, Central Kalimantan, Indonesia. Biodiversitas, 26(3), 4218–4227. https://doi.org/10.13057/biodiv/d260315
Libretexts. (2024, October 28). 22.2: Diversity indices. Biology LibreTexts. https://bio.libretexts.org/Courses/Gettysburg_College/01%3A_Ecology_for_All/22%3A_Biodiversity/22.02%3A_Diversity_Indices
Lürling, M., Mendes e Mello, M., van Oosterhout, F., & de Senerpont Domis, L. N. (2018). Response of natural cyanobacteria and algae assemblages to a nutrient pulse and elevated temperature. Frontiers in Microbiology, 9, 1851. https://doi.org/10.3389/fmicb.2018.01851
MDPI. (2023). Assemblage patterns of microalgae along the upstream to downstream gradient of the Okavango Delta: Abundance, taxonomic diversity, and functional diversity. Water, 15(15), 2692. https://doi.org/10.3390/w15152692
Pokhrel, P., Ghimire, L., & Rai, S. (2021). Seasonal variation of algal diversity with reference to water quality in Jagadishpur Reservoir, Nepal. Water, 13(21), 3003. https://doi.org/10.3390/w13213003
Remias, D., Jost, S., Boenigk, J., Wastian, J., & Lütz, C. (2013). Hydrurus‐related golden algae (Chrysophyceae) cause yellow snow in polar summer snowfields. Phycological Research, 61(4), 277–285. https://doi.org/10.1111/pre.12025
Reyes, J. P., Bicaldo, P. G., & Tamayo, A. R. (2024). Spatiotemporal variability of phytoplankton communities in the euphotic layers of a tropical caldera lake (Lake Taal, Batangas, Philippines). Biodiversitas, 26(6), 3302–3314. https://doi.org/10.13057/biodiv/d260635
Sherwood, A. R., Carlile, A. L., Neumann, J. M., Kociolek, J. P., Johansen, J. R., Lowe, R. L., Conklin, K. Y., Presting, G. G. (2014). The Hawaiian freshwater algae biodiversity survey (2009-2014): systematic and biogeographic trends with an emphasis on the macroalgae. BMC Ecology, 14(1). https://doi.org/10.1186/s12898-014-0028-2
Takahashi, T., Sato, M., Toyooka, K., & Nozaki, H. (2014). Surface ornamentation of Cyanophora paradoxa (Cyanophorales, Glaucophyta) cells as revealed by ultra-high resolution field emission scanning electron microscopy. CYTOLOGIA, 79(1), 119–123. https://doi.org/10.1508/cytologia.79.119
Van Leeuwe, M. A., Tedesco, L., Arrigo, K. R., Assmy, P., Campbell, K., Meiners, K. M., ... & Stefels, J. (2020). Annual patterns in phytoplankton phenology in Antarctic coastal waters explained by environmental drivers. Limnology and Oceanography, 65(9), 2125–2142. https://doi.org/10.1002/lno.11477
Wang, Q., Gao, Y., Song, X., Wang, Z., & Zhang, X. (2022). Exploring the key factors affecting the seasonal variation of phytoplankton in the coastal Yellow Sea. Frontiers in Marine Science, 9, 1076975. https://doi.org/10.3389/fmars.2022.1076975
Wang, W., Li, Z., & Huang, C. (2022). Heavy metals exacerbate the effect of temperature on the growth of Chlorella sp.: Implications on algal blooms and management. Processes, 10(12), 2638. https://doi.org/10.3390/pr10122638
Wang, Y., Feng, L., Hou, X. (2023). Algal blooms in lakes in China over the past two decades: Patterns, trends, and drivers. Water Resources Research, 59(10), e2022WR033340. https://doi.org/10.1029/2022WR033340
Watanabe, S., & Lewis, L. A. (2017). Phylogenetic interpretation of light and electron microscopic features of selected members of the phylogroup Moewusinia (Chlorophyceae), with new generic taxonomy. Phycologia, 56(3), 329–353. https://doi.org/10.2216/16-64.1
Wiley Online Library. (2023). The importance of integrating phycological research, teaching, outreach, and engagement in a changing world. Journal of Phycology. https://doi.org/10.1111/jpy.13507
Downloads
Published
Issue
Section
License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.